ABSTRACT
Objective To explore the modulation effect of macrophage polarization and tumor microenvironment by colony-stimulating factor 1 receptor (CSF-1R) inhibitor administration in mice colon carcinoma tissue.Methods 24 mice were divided into normal control group,CAC group,CAC + GW2580 group.On day 64,the mice in CAC + GW2580 group were given GW2580 by daily gavage for a week.On 71 st day,the colon tissues were collected.The expression phenotype of macrophage polarization was detected by flow cytometry,the expression of macrophage-associated protein was detected by Western blot,and the expression level of various cytokine mRNAs were detected by PCR.Results The administration of CSF-1R inhibitors in CAC mice resulted in a significant reduction in the percentage of macrophages and M2 cells and a significant increase in M1 (P ＜ 0.05).The expression of M1-related proteins CD86 was up-regulated and the expression of M2-related proteins CD206 was down-regulated (P ＜0.05).Further more,the expression of M2-related IL-4,IL-10,Arg-1 were up-regulated (all P ＜ 0.05),and the expression of M1-related IL-12,iNOS were down-regulated (all P ＜ 0.05).Conclusion CSF-1R inhibitor can effectively modulate macrophage polarization from M2 to M1 in colon tumor and transform the tumor microenvironment from immunosuppression to immunostimulation,exerting tumor inhibition.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of differents solution environments on the ceramic membrane microfiltration of model system of Chinese medicines.</p><p><b>METHOD</b>Taking binary system of soybean protein-berberine as the research object, flux, transmittance of berberine and traping rate of protein as indexes, different solution environment on membrane process were investigated.</p><p><b>RESULT</b>When the concentration of soybean protein was under 1 g x L(-1), the membrane flux was minimum with the traping of berberine decreased slightly as the concentration increased. When pH was 4, the flux was maximum with the traping rate of protein was 99%, and the transmittance of berberine reached above 60%.</p><p><b>CONCLUSION</b>The efficiency of membrane separation can be improved by optimizing the solution environment of water-extraction of chinese medicines. The efficiency of membrane separation is the best when adjust the pH to the isoelectric point of proteins for the proteins as the main pollutant in aqueous solution.</p>